Description
Product Overview
Azura Genomics Reverse Transcriptase is a high-performance enzyme designed for molecular biology applications, including 1-step and 2-step RT-qPCR, array labeling, cDNA libraries, and 3′ and 5′ RACE. It synthesizes cDNA from 100 bp to over 12 Kb, offering reduced RNase H activity for more representative full-length yields. The enzyme maintains sensitivity across a broad template range, from 1 pg to 5 µg of total RNA, and exhibits exceptional thermostability, retaining more than 90% activity after incubation at 50°C for 60 minutes. Manufactured by Azura Genomics, this product is ideal for demanding laboratory environments where precision and reliability are critical.
Usage
This reverse transcriptase is suited for research laboratories, diagnostic facilities, and academic institutions focusing on gene expression analysis, pathogen detection, and functional genomics. It is commonly used in RT-qPCR assays for quantifying RNA levels, in microarray experiments for probe generation, and in constructing cDNA libraries for sequencing projects. Target users include molecular biologists, virologists, and biotechnologists who require robust cDNA synthesis under varying RNA input conditions and thermal stability for extended reactions.
Why Choose Us
Azura Genomics Reverse Transcriptase stands out due to its optimized formulation that minimizes RNase H activity, ensuring higher yields of full-length cDNA and reducing artifacts in downstream applications. The enzyme’s thermostability allows for flexible incubation conditions without loss of efficiency, while its broad sensitivity range accommodates diverse sample types, from low-abundance transcripts to high-input RNA. Backed by Azura Genomics’ commitment to quality, this product undergoes rigorous testing to deliver consistent performance, making it a trusted choice for reproducible results in critical experiments.
Key Features
- High-yield cDNA synthesis with reduced RNase H activity for full-length products up to 12 Kb
- Broad sensitivity range, working reliably with 1 pg to 5 µg of total RNA template
- Excellent thermostability, maintaining over 90% activity after 60 minutes at 50°C
- Versatile applications in RT-qPCR, array labeling, cDNA libraries, and RACE techniques
- Consistent performance and reproducibility for accurate molecular biology results
FAQ
What applications is this reverse transcriptase suitable for?
It is ideal for 1-step and 2-step RT-qPCR, array labeling, cDNA library construction, and 3′ and 5′ RACE, supporting a wide range of molecular biology workflows.
How does the reduced RNase H activity benefit cDNA synthesis?
Reduced RNase H activity minimizes degradation of RNA-DNA hybrids, leading to more representative full-length cDNA and improved yields for long transcripts.
What is the thermostability of this enzyme?
The enzyme retains greater than 90% of its activity after incubation at 50°C for 60 minutes, allowing for stable performance in extended or high-temperature reactions.
Can it handle low RNA inputs?
Yes, it provides reproducible cDNA synthesis from as little as 1 pg of total RNA, making it suitable for samples with limited material.
Is this product compatible with various RNA templates?
It works effectively with a broad range of RNA templates, including total RNA, and is optimized for sensitivity and consistency across different input amounts.
Reviews
There are no reviews yet.